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Abstract: The cumulative expression of multistage deformation is complex multiperiod fractures, which are commonly seen in tectonic zones. The Middle Triassic Leikoupo Formation in the western Sichuan Basin Depression, China, is a typical marine carbonate reservoir with natural fractures caused mainly by tectonic movements. According to outcrops, drill cores, image logging, and fluid inclusions, the fracture characteristics, types of natural fractures, and interactions of fractures are determined. In total, 419 natural fractures in 493.2 m of cores from 7 wells are investigated, which are mainly shear and tensile fractures with a small number of weathering generated fractures. Meanwhile, the results of the stable isotope analysis of δ13C and δ18O, as well as the flow fluid inclusion data, reveal four tectonic periods of fractures with different occurrences. Based on the history of regional tectonic evolution, indicating one period of weathering fractures ascribable to stratal uplift and three periods of structural fractures related to the sequential tectonic movements of the Longmenshan fault belt. By analyzing the interaction relationships of fractures, three types of fracture interaction relationships are observed: cutting, restraining, and overlapping. The four stages fractures are chronologically assigned to (1) the early Indosinian N-S trending compression, (2) the late Indosinian NW-SE compression, (3) the middle Yanshanian NE-SE compression, and (4) the early Himalayan E-W compression. The influence of natural fractures on gas migration and well production in marine carbonates is discussed, and indicates that tectonic fractures could provide seepage channels for gas migration and accumulation from near or distant hydrocarbon source rocks into the Middle Triassic Leikoupo Formation. This study utilizes a pragmatic approach for understanding the fracture genesis mechanism in oil and gas field with multiperiod fractures.