The study on Lower Cambrian dolostones in Tarim Basin can improve our understanding of ancient and deeply buried carbonate reservoirs. In this research, diagenetic fluid characteristics and their control on porosity evolution have been revealed by studying the petrography and in situ geochemistry of different dolomites. Three types of diagenetic fluids were identified: (1) Replacive dolomites were deviated from shallow burial dolomitizing fluids, which might probably be concentrated ancient seawater at early stage. (2) Fine-to-medium crystalline, planar-e diamond pore-filling dolomites (Fd1) were likely slowly and sufficiently crystallized from deep-circulating crustal hydrothermal fluids during Devonian. (3) Coarse crystalline, non-planar-a saddle pore-filling dolomites (Fd2) might rapidly and insufficiently crystallize from magmatic hydrothermal fluids during Permian. Early dolomitizing fluids did not increase the porosity, but transformed the primary pores to dissolution pores through dolomitization. Deep-circulating crustal hydrothermal fluids significantly increased porosity in the early stages by dissolving and then slightly decreased the porosity in the late stage due to Fd1 precipitation. Magmatic hydrothermal fluids only precipitated the Fd2 dolomites and slightly decreased the porosity. In summary, Devonian deep-circulating crustal hydrothermal fluids dominated the porosity evolution of the Lower Cambrian dolostone reservoir in the Tarim Basin.